We found a match
Your institution may have rights to this item. Sign in to continue.
- Title
Disruption of thymopoiesis in ST6Gal I-deficient mice.
- Authors
Julie H Marino; Chibing Tan; Brenda Davis; Eun-Soo Han; Morgan Hickey; Rebecca Naukam; Ashlee Taylor; Kenton S Miller; C Justin Van De Wiele; T Kent Teague
- Abstract
Thymocyte development is accompanied by sequential changes in cell surface glycosylation. For example, medullary thymocytes have increased levels of α2,3-linked sialic acid and a loss of asialo core 1 O-glycans as compared to cortical thymocytes. Some of these changes have been linked to fine tuning of the T cell receptor avidity. We analyzed ST6Gal I transcript abundance and levels of α2,6-linked sialic acid across thymocyte subsets. We found that ST6Gal I transcript levels increased following T cell receptor β-selection suggesting that this sialyltransferase may influence the development of early thymocyte populations. Indeed, low levels of α2,6-linked sialic acid were found in the earliest T lineage cells, and then increased in T cell receptor β-selected cells. To determine whether ST6Gal I influences T cell development, we analyzed ST6Gal I-deficient mice for disruptions in thymocyte populations. We found reduced thymic cellularity in the ST6Gal I-deficient mice starting in the early thymocyte compartments.
- Subjects
T cells; LYMPHOCYTES; CELL membranes; SIALIC acids
- Publication
Glycobiology, 2008, Vol 18, Issue 9, p719
- ISSN
0959-6658
- Publication type
Article
- DOI
10.1093/glycob/cwn051