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- Title
Establishment of method of heat, alkali and ultrasonic synergistic treatment for crushing industrial Saccharomyces cerevisiae.
- Authors
DENG Rui-yun; WANG Kun-yao; REN Xin-ru LIU Rong-jun; CUI Zhi-jing; ZENG Fan-li; LI Li; WANG Zhen
- Abstract
The purpose of the study was to improve the cell wall-breaking rate of industrial Saccharomyces cerevisiae and its application value. The effects of heat, alkali and ultrasonic synergistic treatment on the cell wall-breaking rate of industrial Saccharomyces cerevisiae ScY01 strain were explored. The results showed that the wall-breaking rate of ScY01 strain with a concentration of 6 g/L was 91.78% when treated with heat (boiling water bath 12.5 min), alkali (sodium hydroxide 0.6 g) and ultrasonic crushing (ultrasonic power 450 W, radiation time 11 s, radiation interval 15 s, ultrasonic time 20 min). The amount of protein released after cell fragmentation was used for preliminary application characterization, the total protein release by synergistic treatment was 11.54 times higher than that of alkali alone, 10.26 times higher than that by boiling alone, and 4.01 times higher than that by ultrasonic alone. The study indicates that the synergistic treatment is suitable for model Saccharomyces cerevisiae strain CEN.PK2-1c, and the experimental results can provide a new method for efficient crushing of industrial Saccharomyces cerevisiae cells.
- Subjects
SACCHAROMYCES cerevisiae; ULTRASONICS; ALKALIES; SODIUM hydroxide; STRAIN rate; SACCHAROMYCES
- Publication
Feed Research, 2024, Vol 47, Issue 9, p113
- ISSN
1002-2813
- Publication type
Article
- DOI
10.13557/j.cnki.issn1002-2813.2024.09.020