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- Title
锌转运蛋白 1 siRNA 转染骨髓间充质干细胞对肿瘤坏死因子 α 信号成脂通路的影响
- Authors
章晓云; 黎金焕; 袁振中; 陈跃平; 夏天; 卓映宏; 冯洋; 蓝佼; 董盼锋; 赵斌
- Abstract
BACKGROUND: Previous studies have confirmed that ethanol can promote adipogenic differentiation of bone marrow mesenchymal stem cells (BMSCs), and up-regulate the expression of PPARγ and aP2 in the tumor necrosis factor α (TNF-α) signaling pathway. As a member of the ZIP protein family, Zrt/Irt-like protein 1 (ZIP1) is closely related to bone metabolism and osteogenic differentiation. OBJECTIVE: To study the effect of BMSCs transfected by ZIP1 on TNF-α signaling pathway in the process of adipogenic differentiation. METHODS: The BMSCs from rabbits were isolated and cultured under different concentrations of alcohol (0.03, 0.09, 0.15, 0.21 mol/L), followed by transfection by ZIP1 siRNA and ZIP1 expression vector. RESULTS AND CONCLUSION: After culture in alcohol, the expression levels of aP2 and PPARγ proteins were both significantly increased (P < 0.05), and the level of triglyceride was increased in all alcohol groups except for 0.03 mol/L alcohol group (P < 0.05). After siRNA transfection, the expression levels of aP2 and PPARγ as well as the level of triglyceride were increased significantly in all the alcohol groups (P < 0.05); however, ZIP1 transfection decreased the expression levels of aP2 and PPARγ proteins (P < 0.05). To conclude, ZIP1 siRNA could promote the adipogenic differentiation of BMSCs through the activation of TNF-α signaling pathway.
- Publication
Chinese Journal of Tissue Engineering Research / Zhongguo Zuzhi Gongcheng Yanjiu, 2017, Vol 21, Issue 13, p1986
- ISSN
2095-4344
- Publication type
Article
- DOI
10.3969/j.issn.2095-4344.2017.13.004