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- Title
Development of Simultaneous Derivative Spectrophotometric and HPLC Methods for Determination of 17-Beta-Estradiol and Drospirenone in Combined Dosage Form.
- Authors
Aydoğmuş, Zeynep; Yilmaz, Ece Merve; Yörüsün, Sevgi; Akpinar, Samet
- Abstract
Simple, rapid spectrophotometric, and reverse-phase high performance liquid chromatographic methods were developed for the concurrent analysis of 17-beta-estradiol (ESR) and drospirenone (DRS). The spectrophotometric method was based on the determination of first derivative spectra and determined ESR and DRS using the zero-crossing technique at 208 and 282 nm, respectively, inmethanol. The linear rangewas 0.5-32.0 μgmL-1 forDRS and 0.5-8.0 μgmL-1 for EST.The limit of detection (LOD) values were 0.14 μgmL-1 and 0.10 μgmL-1 and limit of quantification (LOQ) values were 0.42 μgmL-1 and 0.29 μgmL-1 for ESR and DRS, respectively. The chromatographic method was based on the separation of both analytes on a C18 column with a mobile phase containing acetonitrile and water (70 : 30, v/v). Detection was performed with a UV-photodiode array detector at 279 nm. Thelinear range was 0.08-2.5 μgmL-1 for DRS and 0.23-7.5 μgmL-1 for EST. LOD values were 0.05 μgmL-1 and 0.02 μgmL-1 and LOQ values were 0.15 μgmL-1 and 0.05 μgmL-1 for ESR and DRS, respectively. These recommended methods have been applied for the simultaneous determination of ESR and DRS in their tablets.
- Subjects
COMBINATION drug therapy; ESTRADIOL; HIGH performance liquid chromatography; SPECTROPHOTOMETRY
- Publication
International Scholarly Research Notices, 2015, p1
- ISSN
2356-7872
- Publication type
Article
- DOI
10.1155/2015/534065