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- Title
The Potential of a Surface-Modified Titanium Implant with Tetrapeptide for Osseointegration Enhancement.
- Authors
Syam, Syamsiah; Wu, Chia-Jen; Lan, Wen-Chien; Ou, Keng-Liang; Huang, Bai-Hung; Lin, Yu-Yeong; Saito, Takashi; Tsai, Hsin-Yu; Chuo, Yen-Chun; Yen, Ming-Liang; Liu, Chung-Ming; Hou, Ping-Jen; Chaushu, Gavriel
- Abstract
In this study, the innovative dip-coating technique treated titanium (IDCT-Ti) implant with tetrapeptide Gly-Arg-Gly-Asp (GRGD) coating was investigated for its potential to enhance osseointegration. The L929 fibroblast cells were cultured in different concentrations of the GRGD (1%, 2%, and 5%). The cell viability was assessed through 3-[4,5-dimethylthiazol-2-yl]-2,5-diphenyltetrazolium bromide (MTT) assay and live/dead staining. The surface topography and nano-indentation were analyzed by atomic force microscopy. The hemocompatibility was evaluated via field-emission scanning electron microscopy, while contact angle analysis was detected by a goniometer. Radiograph evaluation was determined by panoramic imaging. It was found that the cell growth increased and had a survival rate of more than 70% in 1% GRGD. The mortality of L929 increased with the higher concentration of GRGD. The IDCT-Ti coated with 1% GRGD showed a nano-surface with a Young's modulus that was similar to human cortical bone, and it displayed greater red blood cell accumulations with abundant fibrin formation. As regards the wettability, the IDCT-Ti coated with 1% GRGD was lower than the SLA (sandblasted, large-grit, and acid-etched) treated implant. The X-ray image exhibited no bone loss around the implant at six months after placement. As a result, this study suggests that the IDCT-Ti implant, coated with 1% GRGD, has a tremendous likeliness to enhance osseointegration.
- Subjects
OSSEOINTEGRATION; CONTACT angle; ATOMIC force microscopy; YOUNG'S modulus; ERYTHROCYTES; TITANIUM
- Publication
Applied Sciences (2076-3417), 2021, Vol 11, Issue 6, p2616
- ISSN
2076-3417
- Publication type
Article
- DOI
10.3390/app11062616