We found a match
Your institution may have rights to this item. Sign in to continue.
- Title
Chloro-Substituted Naphthyridine Derivative and Its Conjugate with Thiazole Orange for Highly Selective Fluorescence Sensing of an Orphan Cytosine in the AP Site-Containing Duplexes.
- Authors
Wang, Chun-xia; Sato, Yusuke; Sugimoto, Takashi; Teramae, Norio; Nishizawa, Seiichi
- Abstract
Fluorescent probes with the binding selectivity to specific structures in DNAs or RNAs have gained much attention as useful tools for the study of nucleic acid functions. Here, chloro-substituted 2-amino-5,7-dimethyl-1,8-naphthyridine (ClNaph) was developed as a strong and highly selective binder for target orphan cytosine opposite an abasic (AP) site in the DNA duplexes. ClNaph was then conjugated with thiazole orange (TO) via an alkyl spacer (ClNaph–TO) to design a light-up probe for the detection of cytosine-related mutations in target DNA. In addition, we found the useful binding and fluorescence signaling of the ClNaph–TO conjugate to target C in AP site-containing DNA/RNA hybrid duplexes with a view toward sequence analysis of microRNAs.
- Subjects
NUCLEIC acids; THIAZOLE derivatives; DNA structure; ORPHANS; FLUORESCENCE; CYTOSINE; DNA primers; RIBOSOMAL DNA
- Publication
Applied Sciences (2076-3417), 2020, Vol 10, Issue 12, p4133
- ISSN
2076-3417
- Publication type
Article
- DOI
10.3390/app10124133