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- Title
p53 overexpression is different in Epstein–Barr virus-associated and Epstein–Barr virus-negative carcinoma.
- Authors
Leung, S Y; Chau, K Y; Yuen, S T; Chu, K M; Branicki, F J; Chung, L P
- Abstract
Aims Epstein–Barr virus (EBV) is associated with a subset of gastric and head and neck carcinomas. While p53 mutation and overexpression is common in gastric cancer, in nasopharyngeal carcinoma p53 is overexpressed yet mutation is uncommon, leading to a proposed viral mechanism of p53 upregulation. We examined the expression of p53 protein in 18 EBV-associated gastric carcinomas (EBV-GA) and compared it with 29 age and sex matched EBV-negative gastric carcinomas (EBV0-GA) and 23 non-nasopharyngeal EBV-associated carcinomas (EBV-CAs) arising from various head and neck regions. Methods and results Using two monoclonal antibodies (DO7 and PAb1801) with microwave pre-treatment, the p53 protein was scored according to the intensity and percentage of positive cells. The EBV0-GA showed a clear cut bimodal distribution of p53 levels, with either homogeneous intense staining of most tumour nuclei, or only very weak expression in a few cells. Nearly all the EBV-GA and EBV-CAs showed a weak to moderate p53 expression, characterized by heterogeneous intensity of staining in a variable proportion of tumour cells. Conclusions The difference in p53 levels in the EBV0-GA and EBV-GA is statistically significant. The heterogeneous level of p53 in the EBV-GA and EBV-CAs and its difference from the EBV0-GA is suggestive of a nonmutational mechanism of p53 upregulation and underscores the role of the virus in the oncogenic pathway.
- Subjects
P53 antioncogene; EPSTEIN-Barr virus; CANCER; IMMUNOHISTOCHEMISTRY
- Publication
Histopathology, 1998, Vol 33, Issue 4, p311
- ISSN
0309-0167
- Publication type
Article