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- Title
Side population cells expressing ABCG2 in human adult dental pulp tissue.
- Authors
Honda, M. J.; Nakashima, F.; Satomura, K.; Shinohara, Y.; Tsuchiya, S.; Watanabe, N.; Ueda, M.
- Abstract
Aim To investigate the presence of side population (SP) cells by the Hoechst exclusion method in human adult dental pulp tissue. Methodology Human adult dental pulp-derived cells were generated from third molar teeth. The cells were stained with Hoechst 33342 and sorted into SP cells or non-SP cells [main population (MP) cells]. Both cell types were compared with cell growth and RT–PCR analyses. Results SP cells that express ABCG2, Nestin, Notch-1 and α-smooth muscle actin were found at frequencies ranging from 0.67% to 1.02%. This SP profile disappeared in the presence of verapamil. These SP cells expressed dentine sialophosphoprotein and dentine matrix protein-1 when cultured in osteogenic medium. Conclusion Human adult dental pulp tissue contains SP cells that differentiate into odontoblast-like cells.
- Subjects
STEM cells; ATP-binding cassette transporters; DENTAL pulp; CELL growth; THIRD molars; PHOSPHOPROTEINS; VERAPAMIL; ENDODONTICS
- Publication
International Endodontic Journal, 2007, Vol 40, Issue 12, p949
- ISSN
0143-2885
- Publication type
Article
- DOI
10.1111/j.1365-2591.2007.01301.x