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- Title
Multi-photon near-infrared emission saturation nanoscopy using upconversion nanoparticles.
- Authors
Chaohao Chen; Fan Wang; Shihui Wen; Qian Peter Su; Wu, Mike C. L.; Yongtao Liu; Baoming Wang; Du Li; Xuchen Shan; Kianinia, Mehran; Aharonovich, Igor; Toth, Milos; Jackson, Shaun P.; Peng Xi; Dayong Jin
- Abstract
Multiphoton fluorescence microscopy (MPM), using near infrared excitation light, provides increased penetration depth, decreased detection background, and reduced phototoxicity. Using stimulated emission depletion (STED) approach, MPM can bypass the diffraction limitation, but it requires both spatial alignment and temporal synchronization of high power (femtosecond) lasers, which is limited by the inefficiency of the probes. Here, we report that upconversion nanoparticles (UCNPs) can unlock a new mode of near-infrared emission saturation (NIRES) nanoscopy for deep tissue super-resolution imaging with excitation intensity several orders of magnitude lower than that required by conventional MPM dyes. Using a doughnut beam excitation from a 980 nm diode laser and detecting at 800 nm, we achieve a resolution of sub 50 nm, 1/20th of the excitation wavelength, in imaging of single UCNP through 93 μm thick liver tissue. This method offers a simple solution for deep tissue super resolution imaging and single molecule tracking.
- Publication
Nature Communications, 2018, Vol 9, Issue 1, p1
- ISSN
2041-1723
- Publication type
Article
- DOI
10.1038/s41467-018-05842-w