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- Title
EVALUACIÓN DE DOS MÉTODOS DE EXTRACCIÓN DE SEMILLAS DE UCHUVA (Physalis peruviana L.).
- Authors
Orozco Orozco, Luz Fanny; Trillos González, Ofelia; Cotes Torres, José Miguel
- Abstract
In this research we use Physalis peruviana L. seeds obtained from mature open pollination fruits, originated from 10 accessions used in the plant breeding program supported by the Research Group of Plant Breeding in Andean and Tropical Fruits Trees (COLCIENCIAS). This research was carried out to compare the traditional method for seed extraction (by fruit fermentation) with the direct method, in its effect on the seeds viability and presence of pathogens once these are stored under laboratory conditions. In order to evaluate the viability a direct method (test of germination) and an indirect method (test with tetrazolium) was used. For the storage, the presence of pathogens in the seed was evaluated. During this research, we adapted the tetrazolium test protocol for cape gooseberry. The test of germination allow to establish that the emerge seeds percentage is smaller trough the time and the tetrazolium test shows that almost all the seeds stay viable in all the evaluations; which allows us conclude that it is possible to say that cape gooseberry seeds could be in a latency period or lose the vigor. In addition not pathogens were observed in any of the evaluations, and neither during the four later months of storage. There were not signifi- cant differences between the treatments, therefore the implementation of the direct extraction is recommended since it turns out to be a simpler and fast method than the one of fermentation of the cape gooseberry fruits.
- Subjects
GOOSEBERRIES; POLLINATION; PATHOGENIC microorganisms; TETRAZOLIUM; FERMENTATION
- Publication
Revista Facultad de Ciencias Basicas, 2010, Vol 6, Issue 1, p52
- ISSN
1900-4699
- Publication type
Article