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- Title
Comparison of human adult stem cells from adipose tissue and bone marrow in the treatment of experimental autoimmune encephalomyelitis.
- Authors
Semon, Julie A.; Maness, Catherine; Xiujuan Zhang; Sharkey, Steven A.; Beuttler, Marc M.; Shah, Forum S.; Pandey, Amitabh C.; Gimble, Jeffrey M.; Shijia Zhang; Scruggs, Brittni A.; Strong, Amy L.; Strong, Thomas A.; Bunnell, Bruce A.
- Abstract
Introduction While administration of ex vivo culture-expanded stem cells has been used to study immunosuppressive mechanisms in multiple models of autoimmune diseases, less is known about the uncultured, non-expanded stromal vascular fraction (SVF) based therapy. The SVF is composed of a heterogeneous population of cells and has been used clinically to treat acute and chronic diseases, alleviating symptoms in a range of tissues and organs. Methods In this study, the ability of human SVF cells was compared to culture expanded adipose stem cells (ASCs) and bone-derived marrow stromal cells (BMSCs) as a treatment of myelin oligodendrocyte glycoprotein (MOG)35-55 induced experimental autoimmune encephalitis (EAE) in C57Bl/6J mice, a well-studied multiple sclerosis model (MS). A total of 1 × 106 BMSCs, ASCs, or SVF cells were administered intraperitoneally concomitantly with the induction of disease. Mice were monitored daily for clinical signs of disease by three independent, blinded investigators and rated on a scale of 0 to 5. Spinal cords were obtained after euthanasia at day 30 and processed for histological staining using luxol fast blue (LFB), toluidine blue (TB), and hematoxylin and eosin (H&E) to measure myelin and infiltrating immune cells. Blood was collected from mice at day 30 and analyzed by ELISA to measure serum levels of inflammatory cytokines. Results The data indicate that the intraperitoneal administration of all cell types significantly ameliorates the severity of disease. Furthermore, the data also demonstrates, for the first time, that the SVF was as effective as the more commonly cultured BMSCs and ASCs in an MS model. All cell therapies also demonstrated a similar reduction in tissue damage, inflammatory infiltrates, and sera levels of IFNγ and IL-12. While IFNγ levels were reduced to comparable levels between treatment groups, levels of IL-12 were significantly lower in SVF-treated than BMSC-treated or ASC-treated mice. Conclusions Based on these data, it is evident that the SVF cells have relevant therapeutic potential in an animal model of chronic MS and might represent a valuable tool for stem cell-based therapy in chronic inflammatory disease of the CNS. SVF offers advantages of direct and rapid isolation procedure in a xenobiotic-free environment.
- Subjects
CELLULAR mechanics; PLANT diseases; MULTIPLE sclerosis; BONE marrow; STEM cells
- Publication
Stem Cell Research & Therapy, 2014, Vol 5, Issue 1, p1
- ISSN
1757-6512
- Publication type
Article
- DOI
10.1186/scrt391