We found a match
Your institution may have rights to this item. Sign in to continue.
- Title
De novo peptide sequencing and quantitative profiling of complex protein mixtures using mass-coded abundance tagging.
- Authors
Cagney, Gerard; Emili, Andrew
- Abstract
Proteomic studies require efficient, robust, and practical methods of characterizing proteins present in biological samples. Here we describe an integrated strategy for systematic proteome analysis based on differential guanidination of C-terminal lysine residues on tryptic peptides followed by capillary liquid chromatography-electrospray tandem mass spectrometry. The approach, termed mass-coded abundance tagging (MCAT), facilitates the automated, large-scale, and comprehensive de novo determination of peptide sequence and relative quantitation of proteins in biological samples in a single analysis. MCAT offers marked advantages as compared with previously described methods and is simple, economic, and effective when applied to complex proteomic mixtures. MCAT is used to identify proteins, including polymorphic variants, from complex mixtures and measure variation in protein levels from diverse cell types.
- Subjects
PROTEOMICS; NUCLEOTIDE sequence
- Publication
Nature Biotechnology, 2002, Vol 20, Issue 2, p163
- ISSN
1087-0156
- Publication type
Article
- DOI
10.1038/nbt0202-163