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- Title
Neuroprotective Effects of 3α-Acetoxyeudesma-1,4(15),11(13)-trien-12,6α-olide Against Dopamine-Induced Apoptosis in the Human Neuroblastoma SH-SY5Y Cell Line.
- Authors
Koo, Uk; Nam, Kung-Woo; Ham, Ahrom; Lyu, Dahyun; Kim, Bora; Lee, Sung-Jin; Kim, Kyeong; Oh, Ki-Bong; Mar, Woongchon; Shin, Jongheon
- Abstract
Dopamine (DA), as a neurotoxin, can elicit severe Parkinson's disease-like syndrome by elevating intracellular reactive oxygen species (ROS) levels and apoptotic activity. We examined the inhibitory effects of 3α-acetoxyeudesma-1,4(15),11(13)-trien-12,6α-olide (AETO), purified from the leaves of Laurus nobilis L., on DA-induced apoptosis and α-synuclein (α-syn) formation in dopaminergic SH-SY5Y cells. AETO decreased the active form of caspase-3 and the levels of p53, which were accompanied by increased levels of Bcl-2 in a dose-dependent manner. Flow cytometric and Western blot analysis showed that AETO significantly inhibited DA-induced apoptosis along with suppression of intracellular tyrosinase activity, ROS generation, quinoprotein, and α-syn formation ( P < 0.01). These results indicate that AETO inhibited DA-induced apoptosis, which is closely related to the suppression of intracellular tyrosinase activity and the formation of α-syn, ROS, and quinoprotein in SH-SY5Y cells.
- Subjects
NEUROPROTECTIVE agents; DOPAMINE; APOPTOSIS; NEUROBLASTOMA; CELL lines; NEUROTOXIC agents; PHENOL oxidase; QUINOPROTEINS
- Publication
Neurochemical Research, 2011, Vol 36, Issue 11, p1991
- ISSN
0364-3190
- Publication type
Article
- DOI
10.1007/s11064-011-0523-1