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- Title
Heat stress increases insulin sensitivity in pigs.
- Authors
Sanz Fernandez, M. Victoria; Stoakes, Sara K.; Abuajamieh, Mohannad; Seibert, Jacob T.; Johnson, Jay S.; Horst, Erin A.; Rhoads, Robert P.; Baumgard, Lance H.
- Abstract
Proper insulin homeostasis appears critical for adapting to and surviving a heat load. Further, heat stress ( HS) induces phenotypic changes in livestock that suggest an increase in insulin action. The current study objective was to evaluate the effects of HS on whole-body insulin sensitivity. Female pigs (57 ± 4 kg body weight) were subjected to two experimental periods. During period 1, all pigs remained in thermoneutral conditions ( TN; 21°C) and were fed ad libitum. During period 2, pigs were exposed to: (i) constant HS conditions (32°C) and fed ad libitum ( n = 6), or (ii) TN conditions and pair-fed ( PFTN; n = 6) to eliminate the confounding effects of dissimilar feed intake. A hyperinsulinemic euglycemic clamp ( HEC) was conducted on d3 of both periods; and skeletal muscle and adipose tissue biopsies were collected prior to and after an insulin tolerance test ( ITT) on d5 of period 2. During the HEC, insulin infusion increased circulating insulin and decreased plasma C-peptide and nonesterified fatty acids, similarly between treatments. From period 1 to 2, the rate of glucose infusion in response to the HEC remained similar in HS pigs while it decreased (36%) in PFTN controls. Prior to the ITT, HS increased (41%) skeletal muscle insulin receptor substrate-1 protein abundance, but did not affect protein kinase B or their phosphorylated forms. In adipose tissue, HS did not alter any of the basal or stimulated measured insulin signaling markers. In summary, HS increases whole-body insulin-stimulated glucose uptake.
- Subjects
PHYSIOLOGICAL effects of heat; INSULIN resistance; LABORATORY swine; SKELETAL muscle; ADIPOSE tissues; FREE fatty acids; PROTEIN kinase B; GLUCOSE in the body
- Publication
Physiological Reports, 2015, Vol 3, Issue 8, pn/a
- ISSN
2051-817X
- Publication type
Article
- DOI
10.14814/phy2.12478