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- Title
MOLECULAR CHARACTERIZATION OF THE GENE ENCODING CHECKPOINT HOMOLOG 1 (CHK1) IN DAPHNIOPSIS TIBETANA SARS (CRUSTACEA: CLADOCERA) AND ITS EXPRESSION AT DIFFERENT REPRODUCTIVE STAGES.
- Authors
YIN, D. P.; ZHAO, W.; ZHANG, Y.; WEI, J.; WANG, S.; BAO, X. B.
- Abstract
Checkpoint homolog 1 (encoded by Chk1) plays an important role in the growth and development of water flea. In this study, the full-length cDNA of Chk1 encoding an aquaporin from Daphniopsis tibetana was isolated by rapid amplification of cDNA ends-PCR (GenBank ID. MW561428.1). The DtChk1 cDNA was 2025 bp long with a 318-bp 5'- untranslated region (UTR) and a 216-bp 3'-UTR’ containing a stop codon (TGA). DtChk1 cDNA was 1494 bp in open reading frame and encoded a 497-aa polypeptide. At the amino acid sequence level, DtChk1 showed high homology with homologs in Daphnia magna (78%), Daphnia carinata (77%), and Daphnia pulex (73%). Its homology with Chk1 sequences from other species ranged from 26% to 45%. Comparison of the deduced amino acid sequence of DtChk1 with homologs in other species revealed a high degree of conservation of the residues and domains that are essential for biological evolution. Using real-time quantitative PCR, we investigated DtChk1 gene transcript levels in male and female D. tibetana at different reproductive phases. The transcript levels of DtChk1 were significantly higher in sexual males than in parthenogenetic and sexual females (P<0.05), and significantly higher in sexual females than in parthenogenetic females (P<0.05). We will discuss the potential uses of DtChk1 in ecotoxicological studies in the future.
- Subjects
CHECKPOINT kinase 1; GENE expression; BIOLOGICAL evolution; CLADOCERA; AMINO acid sequence
- Publication
Applied Ecology & Environmental Research, 2022, Vol 20, Issue 6, p5263
- ISSN
1589-1623
- Publication type
Article
- DOI
10.15666/aeer/2006_52635275