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- Title
DNA synthesis pattern, proteome, and ABA and GA signalling in developing seeds of Norway maple (Acer platanoides).
- Authors
Staszak, Aleksandra M.; Rewers, Monika; Sliwinska, Elwira; Klupczyńska, Ewelina A.; Pawłowski, Tomasz A.
- Abstract
Mature seeds of Norway maple exhibit desiccation tolerance and deep physiological dormancy. Flow cytometry, proteomics, and immunodetection have been combined to investigate seed development of this species. DNA content analysis revealed that cell cycle/endoreduplication activity differs between seed organs and developmental stages. In the embryo axis, the proportion of the nuclei with the highest DNA content (4C) increases at the beginning of maturation (17 weeks after flowering; WAF), and then is stable until the end of maturation, to increase again after drying. In cotyledons, during maturation endopolyploid nuclei (8C) occur and the intensity of endoreduplication increases up to 21 WAF, and then is stable until development is completed. In dry mature seeds, the proportion of 4C nuclei is high, and reaches 36% in the embryo axis and 52% in cotyledons. Proteomic studies revealed that energy and carbon metabolism, fatty acid biosynthesis, storage and antioxidant proteins are associated with seed development. Study of the ABI5 protein, a transcription factor involved in ABA signalling, and the RGL2 protein, a repressor of the GA signalling indicates that the highest accumulation of these proteins occurs in fully-matured and dried seeds. It is suggested that this increase in accumulation can be associated with completion of maturation, mainly with desiccation and dormancy acquisition. Norway maple embryo (axis and cotyledons) development was studied, including DNA synthesis, proteins, and plant hormone signalling. Results revealed that cell cycle/endoreduplication activity is a good indicator of developmental stages, distinguishing maturation phases, including deposition of storage reserves, desiccation and dormancy acquisition. Our results provide novel insights into the mechanisms underlying the development of temperate tree seeds.
- Subjects
PROTEOMICS; DNA synthesis; ENDOPOLYPLOIDY; PLANT development; ABSCISIC acid
- Publication
Functional Plant Biology, 2019, Vol 46, Issue 2, p152
- ISSN
1445-4408
- Publication type
Article
- DOI
10.1071/FP18074