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- Title
In vitro propagation of Trichosanthes kirilowii Maxim. through nodal segment shoot proliferation.
- Authors
Joo, Su Ji; Yoon, A. Ra; Kim, Yong-Goo; Moon, Byeong Cheol; Komakech, Richard; Kang, Youngmin
- Abstract
Trichosanthes kirilowii Maxim. is a vital traditional herbal medicinal plant found in northeastern Asia. Its roots, fruits, and seeds are used as food and medicine. Roots harvested for medicinal use take over 3 yr to mature when the plant is grown in a traditional way through cultivation in the field. This coupled with uncertainty in identification of the plant when collected from the wild calls for a standard in vitro propagation system to meet the increasing demand for it. The purpose of this study was to develop a standard protocol for the in vitro micropropagation of T. kirilowii. Ten different media supplemented with different concentrations of plant growth regulators were evaluated. At 5 wk, De Greef and Jacobs medium supplemented with 0.1 mg L−1 kinetin led to optimal shoot growth, while the same medium supplemented with 0.5 mg L−1 indole 3-butyric acid induced optimal root growth, also at 5 wk. The micropropagated plants that were acclimatized for 8 wk in the greenhouse produced mature root tubers after planted in the field for 3 mo. Therefore, these findings provide a basis for future large-scale in vitro propagation of T. kirilowii.
- Subjects
ASIA; PLANT regulators; MEDICINAL plants; PLANT identification; TUBERS; ROOT growth; GREENHOUSE plants
- Publication
In Vitro Cellular & Developmental Biology Plant, 2019, Vol 55, Issue 6, p702
- ISSN
1054-5476
- Publication type
Article
- DOI
10.1007/s11627-019-10010-w