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- Title
HPLC fingerprint of Nauclea officinalis stems in different harvesting period.
- Authors
LIU Ai-xia; ZHOU Ming-yan; LI Xiang-yi; LI Li; ZHANG Yu-xin; ZHENG Xiu-wen; WEN Huan; ZHANG Jun-qing; ZHANG Xu-guang
- Abstract
Objective: To establish HPLC fingerprint of Nauclea officinalis stems in different harvesting periods, and analyze the effect of harvest time on the quality of the medicinal materials by combining with chemical pattern recognition. Methods: The analysis was performed on Sun Fire -C18(150 mm×4.6 mm, 5 μm) column. The mobile phase consisted of acetonitrile- 0.1% phosphoric acid solution (gradient elution) at a flow rate of 1.0 mL/min. Results: The HPLC fingerprint of Nauclea officinalis stems was established and 12 common peaks were determined, and 3 chromatographic peaks were identified by comparison with the mixed references. There were some differences in the quality of Nauclea officinalis in different harvesting periods. The OPLS-DA analysis successfully predicted four main markers of quality difference. Conclusion: The established HPLC fingerprint could reflect the composition characteristics of Nauclea officinalis stems in different harvesting period, and the main markers that influence the composition difference of the stems could be used as key indicators for the quality control.
- Subjects
CHEMICAL fingerprinting; HARVESTING; GRADIENT elution (Chromatography); HARVESTING time; QUALITY control
- Publication
Journal of Hainan Medical University, 2023, Vol 29, Issue 7, p8
- ISSN
1007-1237
- Publication type
Article