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- Title
Growth inhibition due to aged silicone rubber tubing.
- Authors
LEGAN, J. D.; OWENS, J. D.
- Abstract
Growth of the obligately methylotrophic Bacterium B6/2 in continuous culture was inhibited when the mineral salts-methylamine medium was supplied through silicone rubber tubing which had been autoclaved more than 50 times. The growth inhibition was removed when the medium was supplied through new silicone rubber tubing. Growth in small-scale batch cultures was delayed or inhibited after the medium passed through the former tubing but not when it had passed through the latter. It is recommended that silicone rubber medium supply lines to chemostats are replaced periodically.
- Publication
Letters in Applied Microbiology, 1987, Vol 5, Issue 4, p75
- ISSN
0266-8254
- Publication type
Article
- DOI
10.1111/j.1472-765X.1987.tb01618.x