We found a match
Your institution may have rights to this item. Sign in to continue.
- Title
Real-time polymerase chain reaction analysis of a 3895-bp mitochondrial DNA deletion in epithelial swabs and its use as a quantitative marker for sunlight exposure in human skin A. Harbottle et al. Measure of acute UV damage in human skin.
- Authors
Harbottle, A.; Maki, J.; Reguly, B.; Wittock, R.; Robinson, K.; Parr, R.; Birch-Machin, M.A.
- Abstract
The use of mitochondrial DNA (mtDNA) damage as a reliable and highly sensitive biomarker of ultraviolet (UV) radiation exposure in both the dermis and epidermis has now been well developed by our group and others. We have previously identified a 3895-bp mtDNA deletion which occurred more frequently and to a higher level in usually sun-exposed skin as opposed to occasionally sun-exposed skin. This work focused on older-aged individuals and, in particular, perilesional, histologically normal skin biopsies taken from patients with skin cancer. To develop novel, less-invasive methods of obtaining skin samples (i.e. epidermis) from volunteers covering a much wider age range and larger number of individuals ( n = 239). The 3895-bp deletion was quantified by a specific real-time polymerase chain reaction assay in normal human epidermis samples taken from three body sites with differing sun exposure. The results show a statistical increase of the level of the 3895-bp deletion with increasing sun exposure in the epidermal swabs of human skin ( P < 0·001) and with increasing age of the donor in the needle biopsy samples. These data suggest that the upper layers of the epidermis are an accessible and reliable site for assessing mtDNA damage caused by UV exposure.
- Subjects
MITOCHONDRIAL DNA; POLYMERASE chain reaction; SKIN cancer; PHYSIOLOGICAL effects of ultraviolet radiation; DNA damage; BIOPSY
- Publication
British Journal of Dermatology, 2010, Vol 163, Issue 6, p1291
- ISSN
0007-0963
- Publication type
Article
- DOI
10.1111/j.1365-2133.2010.10001.x