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- Title
Bet v 1-like pollen allergens of multiple F agales species can sensitize atopic individuals.
- Authors
Hauser, M.; Asam, C.; Himly, M.; Palazzo, P.; Voltolini, S.; Montanari, C.; Briza, P.; Bernardi, M. L.; Mari, A.; Ferreira, F.; Wallner, M.
- Abstract
Summary Background In the temperate climate zone of the Northern hemisphere, F agales pollen allergy represents the main cause of winter/spring pollinosis. Among F agales trees, pollen allergies are strongly associated within the B etulaceae and the F agaceae families. It is widely accepted that F agales pollen allergies are initiated by sensitization against Bet v 1, the birch pollen major allergen, although evidence is accumulating that the allergenic activity of some Bet v 1-like molecules has been underestimated. Objective To investigate the allergenic potential of the clinically most important F agales pollen allergens from birch, alder, hazel, hornbeam, hop-hornbeam, oak, beech and chestnut. Methods To obtain the full spectrum of allergens, the three previously unavailable members of the Bet v 1-family, hop-hornbeam Ost c 1, chestnut Cas s 1 and beech Fag s 1, were identified in the respective pollen extracts, cloned and produced as recombinant proteins in E . coli. Together with recombinant Bet v 1, Aln g 1, Car b 1, Cor a 1 and Que a 1, the molecules were characterized physicochemically, mediator release assays were performed and IgE cross-reactivity was evaluated by ELISA and Immuno Solid-phase Allergen Chip ( ISAC) IgE inhibition assays. Results All allergens showed the typical Bet v 1-like secondary structure elements, and they were all able to bind serum IgE from F agales allergic donors. Strong IgE binding was observed for B etuloideae and C oryloideae allergens, however, cross-reactivity between the two subfamilies was limited as explored by inhibition experiments. In contrast, IgE binding to members of the F agaceae could be strongly inhibited by serum pre-incubation with allergens of the B etuloideae subfamily. Conclusions and Clinical Relevance The data suggest that Bet v 1-like allergens of the B etuloideae and C oryloideae subfamily might have the potential to induce IgE antibodies with different specificities, while allergic reactions towards F agaceae allergens are the result of IgE cross-reactivity.
- Subjects
POLLEN; ALLERGIES; RECOMBINANT proteins; ESCHERICHIA coli; ENZYME-linked immunosorbent assay
- Publication
Clinical & Experimental Allergy, 2011, Vol 41, Issue 12, p1804
- ISSN
0954-7894
- Publication type
Article
- DOI
10.1111/j.1365-2222.2011.03866.x