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- Title
In vitro Ammonia Utilization and Urea Synthesis by Rat Liver after Portacaval Shunt.
- Authors
Sharma, Peeyush; Weichetek, Maria; Karlik, Wojtek; Jeppsson, Bengt; Garwacki, Stanislaw
- Abstract
Background: The objective of this study was to study in vitro ammonia utilization and urea synthesis by the rat liver after portacaval shunt (PCS) in comparison to controls. This study is part of a series of experiments to investigate metabolic derangements in the liver following PCS. Methods: Sixteen male Wistar rats weighing 150-230 g were used. PCS was performed on 8 and the rest served as sham-operated controls. The experiments were performed 4 weeks postoperatively. The usually monitored postoperative parameters after PCS like body weight, rise in ammonia levels in the peripheral blood, atrophy of mesenteric fat, atrophy of the liver, etc., were comparable to other PCS series. Liver slices (1 × 2 × 1 mm) were prepared from each rat liver and incubated in Krebs-Henseleit buffer. Results: After incubation, ammonia utilization in the controls was 162.88 ± 20.12 μmol/g dry weight/h while in PCS rats it was 81.35 ± 2.64 μmol/g dry weight/h (p < 0.0001). The rate of urea synthesis in controls was 122.54 ± 12.93 μmol/g dry weight/h whereas in the PCS group it was 14.99 ± 2.21 μmol/g dry weight/h (p < 0.0001). Conclusion: These results indicate a significant decline in the capacity of the liver for ammonia utilization as well as urea synthesis after PCS. Copyright © 1997 S. Karger AG, Basel
- Publication
Digestion, 1997, Vol 58, Issue 6, p587
- ISSN
0012-2823
- Publication type
Article
- DOI
10.1159/000201506