We found a match
Your institution may have rights to this item. Sign in to continue.
- Title
Circulating tumor DNA is a prognostic biomarker in metastatic melanoma patients treated with chemoimmunotherapy and BRAF inhibitor.
- Authors
Mattila, Kalle E.; Mäkelä, Siru; Kytölä, Soili; Andersson, Emma; Vihinen, Pia; Ramadan, Susan; Skyttä, Tanja; Tiainen, Leena; Vuoristo, Meri-Sisko; Tyynelä-Korhonen, Kristiina; Koivunen, Jussi; Kohtamäki, Laura; Aittomäki, Kristiina; Hernberg, Micaela
- Abstract
Detectable circulating tumor DNA (ctDNA) has been associated with worse prognosis in melanoma patients. We studied plasma ctDNA as a prognostic biomarker in 19 patients with metastatic melanoma and a detectable tumor mutation (13 BRAF, 5 NRAS, and 1 KRAS). Patients had received chemotherapy, interferon-alpha, and vemurafenib in a prospective clinical trial. Mutant allele frequency (MAF %) was determined with droplet digital PCR from pretreatment and sequential plasma samples. Higher pretreatment plasma ctDNA levels (MAF ≥3%) and detectable plasma ctDNA levels (MAF >0%) at the time of radiologically confirmed best objective response were associated with poor prognosis even when accounting for other relevant prognostic factors including performance status, tumor mutation, metastasis stage, and lactate dehydrogenase levels in multivariable analysis. Higher pretreatment plasma ctDNA levels and sustained detectable plasma ctDNA levels during treatment indicated poor prognosis in metastatic melanoma patients.
- Subjects
MELANOMA prognosis; CANCER chemotherapy; MELANOMA; BLOOD plasma; MULTIVARIATE analysis; METASTASIS; ALLELES; TREATMENT effectiveness; CANCER patients; INTERFERONS; TRANSFERASES; DESCRIPTIVE statistics; EXTRACELLULAR space; TUMOR markers; POLYMERASE chain reaction; OXIDOREDUCTASES; NUCLEIC acids; IMMUNOTHERAPY
- Publication
Acta Oncologica, 2022, Vol 61, Issue 10, p1263
- ISSN
0284-186X
- Publication type
Article
- DOI
10.1080/0284186X.2022.2137693