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- Title
Reproducibility of Manual Platelet Estimation Following Automated Low Platelet Counts.
- Authors
Al-Hosni, Zainab S.; Al-Khabori, Murtadha; Al-Mamari, Sahimah; Al-Qasabi, Jamal; Davis, Hiedi; Al-Lawati, Hatim; Al-Riyami, Arwa Z.
- Abstract
Objectives: Manual platelet estimation is one of the methods used when automated platelet estimates are very low. However, the reproducibility of manual platelet estimation has not been adequately studied. We sought to assess the reproducibility of manual platelet estimation following automated low platelet counts and to evaluate the impact of the level of experience of the person counting on the reproducibility of manual platelet estimates. Methods: In this cross-sectional study, peripheral blood films of patients with platelet counts less than 100 x 109/L were retrieved and given to four raters to perform manual platelet estimation independently using a predefined method (average of platelet counts in 10 fields using lOOx objective multiplied by 20). Data were analyzed using intraclass correlation coefficient (ICC) as a method of reproducibility assessment. Results: The ICC across the four raters was 0.840, indicating excellent agreement. The median difference of the two most experienced raters was 0 (range: -64 to 78). The level of platelet estimate by the least-experienced rater predicted the disagreement (p = 0.037). When assessing the difference between pairs of raters, there was no significant difference in the ICC (p = 0.420). Conclusions: The agreement between different raters using manual platelet estimation was excellent. Further confirmation is necessary, with a prospective study using a gold standard method of platelet counts.
- Subjects
BLOOD platelets; PROBABILITY theory; RESEARCH evaluation; INTER-observer reliability; CROSS-sectional method; WORK experience (Employment); DESCRIPTIVE statistics; PLATELET count; INTRACLASS correlation
- Publication
Oman Medical Journal, 2016, Vol 31, Issue 6, p409
- ISSN
1999-768X
- Publication type
Article
- DOI
10.5001/omj.2016.83