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- Title
Correlation of Copper Interaction, Copper-Driven Aggregation, and Copper-Driven H<sub>2</sub>O<sub>2</sub> Formation with Aβ40 Conformation.
- Authors
Chia-Anne Yang; Yung-Han Chen; Shyue-Chu Ke; Yi-Ru Chen; Hsien-Bin Huang; Ta-Hsien Lin; Yi-Cheng Chen
- Abstract
The neurotoxicity of Aβ is associated with the formation of free radical by interacting with redox active metals such as Cu2+. However, the relationship between ion-interaction, ion-driven free radical formation, and Aβ conformation remains to be further elucidated. In the present study, we investigated the correlation of Cu2+ interaction and Cu2+-driven free radical formation with Aβ40 conformation. The Cu2+-binding affinity for Aβ40 in random coiled form is 3-fold higher than that in stable helical form. Unexpectedly but interestingly, we demonstrate in the first time that the stable helical form of Aβ40 can induce the formation of H2O2 by interacting with Cu2+. On the other hand, the H2O2 generation is repressed at Aβ/Cu2+ molar ratio 1 when Aβ40 adopts random coiled structure. Taken together, our result demonstrates that Aβ40 adopted a helical structure that may play a key factor for the formation of free radical with Cu2+ ions.
- Subjects
COPPER metabolism; PROTEIN metabolism; ALZHEIMER'S disease; BIOPHYSICS; HYDROGEN peroxide; RESEARCH methodology; RESEARCH funding; SPECTRUM analysis
- Publication
International Journal of Alzheimer's Disease, 2011, Vol 2011, p1
- ISSN
2090-0252
- Publication type
Article
- DOI
10.4061/2011/607861