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- Title
A staining method for monitoring subgingival bacteria associated with periodontal disease.
- Authors
Quee, Trevor Chin; Bergeron, Marie-Josée; Amsel, Rhonda; Chan, E. C. S.
- Abstract
Dark-field microscopy to monitor subgingival bacteria has been advocated as an accepted adjunct in monitoring periodontal disease. By aging two flagella stains (Gray's and Ryu's) for at least 3 wk at room temperature, we were able to stain four morphological groups, spirochetes, motile rods, coccoids and "other types" which have been suggested as indicator organisms of periodontal destruction. The relative proportions of these groups present in plaque samples from 17 clinically diseased sites were compared using dark-field microscopy and the two staining methods. All three techniques gave similar proportions of spirochetes. However, the staining methods revealed significantly more flagellated cells than motile cells seen with dark-field microscopy. Advantages of the staining method over dark-field microscopy include the convenience of examining plaque samples at a later lime and the availability of a permanent record.
- Subjects
GINGIVAL fluid; BACTERIAL antigens; STAINS &; staining (Microscopy); PERIODONTAL disease; MEDICAL experimentation on humans; MICROSCOPY
- Publication
Journal of Periodontal Research, 1986, Vol 21, Issue 6, p722
- ISSN
0022-3484
- Publication type
Article
- DOI
10.1111/j.1600-0765.1986.tb01510.x