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- Title
Arbovirus-Derived piRNAs Exhibit a Ping-Pong Signature in Mosquito Cells.
- Authors
Vodovar, Nicolas; Bronkhorst, Alfred W.; Cleef, Koen W. R. van; Miesen, Pascal; Blanc, Hervé; van Rij, Ronald P.; Saleh, Maria-Carla
- Abstract
The siRNA pathway is an essential antiviral mechanism in insects. Whether other RNA interference pathways are involved in antiviral defense remains unclear. Here, we report in cells derived from the two main vectors for arboviruses, Aedes albopictus and Aedes aegypti, the production of viral small RNAs that exhibit the hallmarks of ping-pong derived piwiassociated RNAs (piRNAs) after infection with positive or negative sense RNA viruses. Furthermore, these cells produce endogenous piRNAs that mapped to transposable elements. Our results show that these mosquito cells can initiate de novo piRNA production and recapitulate the ping-pong dependent piRNA pathway upon viral infection. The mechanism of viralpiRNA production is discussed.
- Subjects
SMALL interfering RNA; MOSQUITOES; ANTIVIRAL agents; RNA interference; RNA viruses; AEDES albopictus; AEDES aegypti
- Publication
PLoS ONE, 2012, Vol 7, Issue 1, p1
- ISSN
1932-6203
- Publication type
Article
- DOI
10.1371/journal.pone.0030861