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- Title
Andrology: Acrosome reaction induced in a limited population of human spermatozoa by progesterone (Ca-dependent) and ATP (Ca-independent).
- Authors
Tomiyama, Tatsuhiro; Ohashi, Kazutomo; Tsutsui, Tateki; Saji, Fumitaka; Tanizawa, Osamu
- Abstract
We examined the kinetics of the acrosome reaction induced in human spermatozoa by progesterone (Ca-dependent) and adenosine 5'-triphosphate (ATP; Ca-independent). ATP and progesterone did not induce the acrosome reaction unless spermatozoa were incubated in a capacitation medium. ATP exhibited a constant induction of the acrosome reaction regardless of the incubation period, while progesterone began to induce the acrosome reaction after >6 h of incubation. At 24 h of incubation, the percentages of spermatozoa in which the acrosome reaction had been induced by both progesterone and ATP were almost equal. To determine whether a limited population of human spermatozoa was reactive to both progesterone and ATP, we employed an MH61 bead binding method. When the acrosome-reacted spermatozoa were removed with MH61 beads, their percentage in the sperm suspension was decreased to <3%. At 24 h of incubation, progesterone and ATP induced the acrosome reaction in 12.0 and 10.0% of spermatozoa, respectively. After MH61 bead binding was performed, the remaining spermatozoa did not react to the other activator (progesterone -> ATP, ATP -> progesterone). These findings indicate that only a limited population of human spermatozoa has the potential to undergo the acrosome reaction when stimulated by both progesterone and ATP.
- Publication
Human Reproduction, 1995, Vol 10, Issue 8, p2052
- ISSN
0268-1161
- Publication type
Article