We found a match
Your institution may have rights to this item. Sign in to continue.
- Title
Effects of dibutyryl cyclic guanosine monophosphate on human spermatozoal motility and penetration of zona-free hamster oocytes.
- Authors
Chan, Y. M.; Chan, S. Y. W.; Tucker, M. J.; Wong, C. J. Y.; Leung, C. K. M.; Leong, M. K. H.
- Abstract
The possible effects of N ,2' -O-dibutyryl 3'–5'-cyclic guanosine monophosphate (dbcGMP), imidazole (IM) or their combination on human spermatozoal motility and penetration of zona-free hamster oocytes were investigated. Motility of human spermatozoa was assessed at 0, 0.5, 2, 3, 5 and 8 h over an 8-h incubation period with various concentrations of dbcGMP, IM or dbcGMP plus IM. Both percentage motile spermatozoa and progression of human spermatozoa decreased with increasing concentrations of dbcGMP (12–96 mM) and IM (10–80 mM). This inhibitory effect on motility was more pronounced when dbcGMP was added with IM. For assessment of human spermatozoal penetration into zona-free hamster oocytes, spermatozoa were treated for 30 min with either dbcGMP, IM or both after 5 h of capacitation pre-incubation at 37°C. Alternatively, the compounds were only added during the capacitation preincubation period. At concentrations of dbcGMP ≤ 24 mM, neither of the treatments had a significant effect on the penetration rate of zona-free hamster oocytes in comparison with the controls. However, a higher concentration of dbcGMP (48 mM) inhibited the penetration rate. A similar trend was observed when dbcGMP plus IM were added. IM alone had no significant effect on the penetration rate at the concentrations tested (10–40 mM). Our data indicated an inhibitory effect of dbcGMP on both human spermatozoal motility and penetration into zona-free hamster oocytes.
- Publication
Human Reproduction, 1990, Vol 5, Issue 3, p304
- ISSN
0268-1161
- Publication type
Article