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- Title
荧光标记解淀粉芽孢杆菌 WIKI 在山核桃树体和土壤中的 定殖 规律.
- Authors
商 竞; 方 伟; 顾佳悦; 严淑烟; 邵 帅; 梁辰飞; 秦 华; 陈俊辉; 徐秋芳
- Abstract
In order to determine the colonization dynamics and the best way of Bacillus amylolyticus WIKI inoculation in hickory (Carya cathayensis Sarg. ) and soil, electric shock transformation method was used to introduce green flu orescent protein (GFP) particles to construct the fluorescent labeled strain (GFP-WKI). The liquid GFP-WKI was inoculated by leaf spraying, mot irrigation and trunk injection, and the colonization number of CFP-WKI strain was determined in soil, tots and leaves of hickory at regular intervals during 60 days. The liquid GFP-WKI was added to soil with pH of 5. 6, 6. 8 and 7,9, respectively, in order to find the suitable soil pH for SKIN colonization. The re sults showed that GFP-WKI could be colonized in both soil and inner tissues of hickory trees by leaf spraying, root irrigation and trunk injection, which indicated its good mobility between soil and hickory tree as well as inner tissues. The GFP-WKI was maintained in the range of 10-10 CFU g during 60 days. Compared with root irrigation and leaf spraying, GFP-WKI strain was more stable in hickory tree and soil by trunk injection. The GFP-WKI was more abundant in soil with pH of 6. 8. The colonization number in hickory seedlings decreased in the order of roots > stems > leaves. It was recommended to inoculate GFP-WKI by root irrigation in respect to colonization effect in tissue and the convenience of application in short-term, combined with adjusting soil pH to 6.8. For the long-term consideration, trunk injection was a better choice.
- Publication
Acta Agriculturae Zhejiangensis, 2021, Vol 33, Issue 1, p77
- ISSN
1004-1524
- Publication type
Article
- DOI
10.3969/j.issn.1004-1524.2021.01.10