We found a match
Your institution may have rights to this item. Sign in to continue.
- Title
Expression of polypeptide GalNAc-transferases in hematopoietic stem/progenitor cells
- Authors
Cheng, Lamei; Wang, Xianyou; Tan, Mengqun; Zhou, Xiaoying; Wang, Qiru
- Abstract
The members of the UDP-GalNAc:polypeptide N-acetylgalactosaminyltransferase (pp-GalNAc-T) family, which transfer GalNAc to polypeptide serine and threonine residues, initiate mucin-type O-linked glycosylation. There are at least 13 functionally characterized members of this family in humans, but no studies have been reported of pp-GalNAc-T isoforms in hematopoietic cells. We isolated and purified CD34+ hematopoietic cells from adult bone marrow by magnetic cell sorting and induced them to differentiate into megakaryocytic lineage cells using an optimal combination of hematopoietic growth factors in serum-free liquid medium. RT-PCR revealed that CD34+ cells expressed pp-GalNAc-T1, T2, T3, T4, T6, T7, T10, T11 and T14, but not pp-GalNAc-T8, T9, T12 and T13. The megakaryocytic lineage cells showed significant increases in the expression of pp-GalNAc-T3, T8, T9, T10 and T13, but pp-GalNAc-T11 and T14 became undetectable. In summary, many pp-GalNAc-T isoforms were expressed in CD34+ cells but the expression pattern changed during differentiation into megakaryocytes. The expression patterns of pp-GalNAc-Ts may be necessary to ensure proper O-glycosylation of mucin-type proteins expressed in CD34+ and megakaryocytic cells.
- Subjects
PEPTIDES; HEMATOPOIETIC stem cells; MEGAKARYOCYTES; GLYCOSYLATION
- Publication
Cell Biology International, 2004, Vol 28, Issue 8/9, p635
- ISSN
1065-6995
- Publication type
Article
- DOI
10.1016/j.cellbi.2004.06.001