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- Title
Preparation, Crystallization, and Preliminary X-Ray Diffraction Study of Mutant Carboxypeptidase T Bearing the Hydrophilized Primary Specificity Pocket.
- Authors
Akparov, V. Kh.; Konstantinova, G. E.; Timofeev, V. I.; Kuranova, I. P.
- Abstract
Crystals of mutant carboxypeptidase T from Thermoactinomyces vulgaris (CPT11QG)with amino acid substitutions L211Q, T262S, L254S, and A251S were grown by the hanging-drop vapor-diffusion method. The crystals belong to sp. gr. P6(3)22. The X-ray-diffraction-data set,suitable for the crystal-structure determination at 2.6 Å resolution,was collected from the grown crystals at the ID23-1 beamline of the European Synchrotron Radiation Facility (ESRF, France).
- Subjects
X-ray diffraction; SYNCHROTRON radiation; AMINO acids; CRYSTALS
- Publication
Crystallography Reports, 2021, Vol 66, Issue 3, p476
- ISSN
1063-7745
- Publication type
Article
- DOI
10.1134/S1063774521030020