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- Title
t 6 A and ms 2 t 6 A Modified Nucleosides in Serum and Urine as Strong Candidate Biomarkers of COVID-19 Infection and Severity.
- Authors
Nagayoshi, Yu; Nishiguchi, Kayo; Yamamura, Ryosuke; Chujo, Takeshi; Oshiumi, Hiroyuki; Nagata, Hiroko; Kaneko, Hitomi; Yamamoto, Keiichi; Nakata, Hirotomo; Sakakida, Korin; Kunisawa, Akihiro; Adachi, Masataka; Kakizoe, Yutaka; Mizobe, Takanori; Kuratsu, Jun-ichi; Shimada, Shinya; Nakamori, Yasushi; Matsuoka, Masao; Mukoyama, Masashi; Wei, Fan-Yan
- Abstract
SARS-CoV-2 infection alters cellular RNA content. Cellular RNAs are chemically modified and eventually degraded, depositing modified nucleosides into extracellular fluids such as serum and urine. Here we searched for COVID-19-specific changes in modified nucleoside levels contained in serum and urine of 308 COVID-19 patients using liquid chromatography-mass spectrometry (LC-MS). We found that two modified nucleosides, N6-threonylcarbamoyladenosine (t6A) and 2-methylthio-N6-threonylcarbamoyladenosine (ms2t6A), were elevated in serum and urine of COVID-19 patients. Moreover, these levels were associated with symptom severity and decreased upon recovery from COVID-19. In addition, the elevation of similarly modified nucleosides was observed regardless of COVID-19 variants. These findings illuminate specific modified RNA nucleosides in the extracellular fluids as biomarkers for COVID-19 infection and severity.
- Subjects
COVID-19; NUCLEOSIDES; LIQUID chromatography-mass spectrometry; BIOMARKERS
- Publication
Biomolecules (2218-273X), 2022, Vol 12, Issue 9, p1233
- ISSN
2218-273X
- Publication type
Article
- DOI
10.3390/biom12091233