We found a match
Your institution may have rights to this item. Sign in to continue.
- Title
An epigenome atlas of neural progenitors within the embryonic mouse forebrain.
- Authors
Rhodes, Christopher T.; Thompson, Joyce J.; Mitra, Apratim; Asokumar, Dhanya; Lee, Dongjin R.; Lee, Daniel J.; Zhang, Yajun; Jason, Eva; Dale, Ryan K.; Rocha, Pedro P.; Petros, Timothy J.
- Abstract
A comprehensive characterization of epigenomic organization in the embryonic mouse forebrain will enhance our understanding of neurodevelopment and provide insight into mechanisms of neurological disease. Here we collected single-cell chromatin accessibility profiles from four distinct neurogenic regions of the embryonic mouse forebrain using single nuclei ATAC-Seq (snATAC-Seq). We identified thousands of differentially accessible peaks, many restricted to distinct progenitor cell types or brain regions. We integrated snATAC-Seq and single cell transcriptome data to characterize changes of chromatin accessibility at enhancers and promoters with associated transcript abundance. Multi-modal integration of histone modifications (CUT&Tag and CUT&RUN), promoter-enhancer interactions (Capture-C) and high-order chromatin structure (Hi-C) extended these initial observations. This dataset reveals a diverse chromatin landscape with region-specific regulatory mechanisms and genomic interactions in distinct neurogenic regions of the embryonic mouse brain and represents an extensive public resource of a 'ground truth' epigenomic landscape at this critical stage of neurogenesis. The authors took a multimodal approach to characterize the differential transcriptome and epigenetic landscape between distinct regions of the embryonic mouse forebrain, revealing many unexplored presumptive promoter-enhancer interactions.
- Subjects
PROSENCEPHALON; MICE; NEUROLOGICAL disorders; PROGENITOR cells; CHROMATIN
- Publication
Nature Communications, 2022, Vol 13, Issue 1, p1
- ISSN
2041-1723
- Publication type
Article
- DOI
10.1038/s41467-022-31793-4