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- Title
Shelf life of a refrigerated FNAC(Fine needle aspiration cytology) specimen.
- Authors
Uma, P. K.; Ramani, Pratibha; Sherlin, Herald. J.; Gheena, S.; Jayaraj, Gifrina; Don, K. R.; Santhanam, Archana
- Abstract
Introduction: Fine-needle aspiration cytology (FNAC) procedures may be used for rapid, cost effective, and apt diagnosis with reduced patient morbidity. Anatomical sites most commonly obtained from are breast, thyroid, lymph nodes, and deep soft tissue lesions. The FNAC specimen is usually refrigerated; in case, we need an additional smear or need to use additional stains for diagnosis. The tendency to refrigerate an FNAC specimen after smearing is quite usual, but it is important to know how long an FNAC will provide useful information if refrigerated. The aim of this study was to evaluate the usefulness of a refrigerated FNAC specimen. Materials and Methods: Five FNAC specimens were collected. They were smeared on alternative days till the 15th day. The smears were immediately fixed in alcohol. They were stained by routine hematoxylin and eosin protocol and mounted. The slides were viewed and scored by two observers. The scoring was based on the quality of information provided by the smear on the basis of cellular content, nuclear details, cytoplasmic details, uniformity of spread, and background effect on the diagnosis. Results: There was no significant change in the quality of information produced by the slides smeared on the 15th day, but there was evidence of clumping of cells after a week due to increased viscosity of the FNAC specimen. After a period of 1 month, there was microbial overload of the specimen. Conclusion: The FNAC specimens can be refrigerated for a period of 2 weeks to provide quality information, further which shows clumping of cells and microbial contamination.
- Subjects
CYTOLOGY; MICROBIAL contamination; MICROBIAL cells; NEEDLE biopsy; CYTOLOGICAL techniques
- Publication
Drug Invention Today, 2019, Vol 12, Issue 5, p880
- ISSN
0975-7619
- Publication type
Article