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- Title
The Role of RsmA in the Regulation of Swarming Motility in Serratia marcescens.
- Authors
Ang, Sunny; Horng, Yu-Tze; Shu, Jwu-Ching; Soo, Po-Chi; Liu, Jia-Hurng; Yi, Wen-Chin; Lai, Hsin-Chih; Luh, Kwen-Tay; Ho, Shen-Wu; Swift, Simon
- Abstract
Swarming motility is a multicellular phenomenon comprising population migration across surfaces by specially differentiated cells. In Serratia marcescens, a network exists in which the flhDC flagellar regulatory master operon, temperature, nutrient status, and quorum sensing all contribute to the regulation of swarming motility. In this study, the rsmA (repressor of secondary metabolites) gene (hereafter rsmA[sub Sm] ) was cloned from S. marcescens. The presence of multicopy, plasmid-encoded rsmA[sub Sm] expressed from its native promoter in S. marcescens inhibits swarming. Synthesis of N-acylhomoserine lactones, presumably by the product of smaI (a luxI homolog isolated from S. marcescens), was also inhibited. Knockout of rsmA[sub Sm] on the S. marcescens chromosome shortens the time before swarming motility begins after inoculation to an agar surface. A single copy of the chromosomal PrsmA[sub Sm] ::luxAB reporter of rsmA[sub Sm] transcription was constructed. Using this reporter, the roles of the flhDC flagellar regulatory master operon, temperature and autoregulation in the control of rsmA[sub Sm] expression were determined. Our findings indicate that RsmA[sub Sm] is a component of the complex regulatory network that controls swarming.Copyright © 2001 National Science Council, ROC and S. Karger AG, Basel
- Subjects
SERRATIA marcescens; GENE expression; REGULATION of cell metabolism; MOTILITY of bacteria; CLONING; CELL physiology; GENETICS
- Publication
Journal of Biomedical Science, 2001, Vol 8, Issue 2, p160
- ISSN
1021-7770
- Publication type
Article
- DOI
10.1159/000054028