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- Title
Structure determination of noncanonical RNA motifs guided by <sup>1</sup>H NMR chemical shifts.
- Authors
Sripakdeevong, Parin; Cevec, Mirko; Chang, Andrew T; Erat, Michèle C; Ziegeler, Melanie; Zhao, Qin; Fox, George E; Gao, Xiaolian; Kennedy, Scott D; Kierzek, Ryszard; Nikonowicz, Edward P; Schwalbe, Harald; Sigel, Roland K O; Turner, Douglas H; Das, Rhiju
- Abstract
Structured noncoding RNAs underlie fundamental cellular processes, but determining their three-dimensional structures remains challenging. We demonstrate that integrating 1H NMR chemical shift data with Rosetta de novo modeling can be used to consistently determine high-resolution RNA structures. On a benchmark set of 23 noncanonical RNA motifs, including 11 'blind' targets, chemical-shift Rosetta for RNA (CS-Rosetta-RNA) recovered experimental structures with high accuracy (0.6-2.0 Å all-heavy-atom r.m.s. deviation) in 18 cases.
- Subjects
NON-coding RNA; MOLECULAR structure of RNA; MOLECULAR structure of catalytic RNA; PROTEIN folding; CHEMICAL shift (Nuclear magnetic resonance); NUCLEAR magnetic resonance spectroscopy
- Publication
Nature Methods, 2014, Vol 11, Issue 4, p413
- ISSN
1548-7091
- Publication type
Article
- DOI
10.1038/nmeth.2876