We found a match
Your institution may have rights to this item. Sign in to continue.
- Title
Segregation of molecules at cell division reveals native protein localization.
- Authors
Landgraf, Dirk; Okumus, Burak; Chien, Peter; Baker, Tania A; Paulsson, Johan
- Abstract
We introduce a nonintrusive method exploiting single-cell variability after cell division to validate protein localization. We found that Clp proteases, widely reported to form biologically relevant foci, were uniformly distributed in Escherichia coli cells, and that many commonly used fluorescent proteins caused severe mislocalization when fused to homo-oligomers. Retagging five other reportedly foci-forming proteins with the most monomeric fluorescent protein tested suggests that the foci were caused by the fluorescent tags.
- Subjects
CELL division; PROTEOLYTIC enzymes; ESCHERICHIA coli; BACTERIAL cells; FLUORESCENT proteins; OLIGOMERS; IMMUNOFLUORESCENCE
- Publication
Nature Methods, 2012, Vol 9, Issue 5, p480
- ISSN
1548-7091
- Publication type
Article
- DOI
10.1038/nmeth.1955