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- Title
Stress‐sensitive dynamics of miRNAs and Elba1 in Drosophila embryogenesis.
- Authors
Örkenby, Lovisa; Skog, Signe; Ekman, Helen; Gozzo, Alessandro; Kugelberg, Unn; Ramesh, Rashmi; Magadi, Srivathsa; Zambanini, Gianluca; Nordin, Anna; Cantú, Claudio; Nätt, Daniel; Öst, Anita
- Abstract
Early‐life stress can result in life‐long effects that impact adult health and disease risk, but little is known about how such programming is established and maintained. Here, we show that such epigenetic memories can be initiated in the Drosophila embryo before the major wave of zygotic transcription, and higher‐order chromatin structures are established. An early short heat shock results in elevated levels of maternal miRNA and reduced levels of a subgroup of zygotic genes in stage 5 embryos. Using a Dicer‐1 mutant, we show that the stress‐induced decrease in one of these genes, the insulator‐binding factor Elba1, is dependent on functional miRNA biogenesis. Reduction in Elba1 correlates with the upregulation of early developmental genes and promotes a sustained weakening of heterochromatin in the adult fly as indicated by an increased expression of the PEV wm4h reporter. We propose that maternal miRNAs, retained in response to an early embryonic heat shock, shape the subsequent de novo heterochromatin establishment that occurs during early development via direct or indirect regulation of some of the earliest expressed genes, including Elba1. Synopsis: Heat shock during Drosophila embryogenesis results in the retention of maternal miRNAs and the downregulation of pre‐MBT genes, including Elba1, which may contribute to setting the stage for subsequent gene expression.Heat shock before the midblastula transition (MBT) results in the retention of maternal miRNAs, which are inversely correlated with a subset of pre‐MBT genes, including Elba1.Elba1 is enriched at the miRNA RISC, and its downregulation following heat shock is Dicer‐1‐dependent.Elba1‐binding sites are found at specific heat shock‐induced genes.Elba1‐3 act as Su(var)s for wm4h.
- Subjects
GENE expression; DROSOPHILA; MICRORNA; EMBRYOLOGY; SPECIFIC heat
- Publication
Molecular Systems Biology, 2023, Vol 19, Issue 5, p1
- ISSN
1744-4292
- Publication type
Article
- DOI
10.15252/msb.202211148