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- Title
Expression ofBrassica napusL.γ-Glutamylcysteine Synthetase and Low-and High-Affinity Sulfate Transporters in Response to Excess Cadmium.
- Authors
Xin Sun; Xue-Mei Sun; Zhi-Min Yang; Shao-Qiong Li; Jin Wang; Song-Hua Wang
- Abstract
In both the roots and leaves ofBrassica napusL. cv. Youyan No. 8 under treatment with 30μmol/L Cd, massive production of non-protein thiols (NPT; mainly containing glutathione (GSH) and phytochelatins (PCs)) was induced, together with an increase inγ-glutamylcysteine synthetase (γ-ECS) mRNA transcripts. Becauseγ-ECS is the key enzyme catalyzing the first step in GSH biosynthesis, which, in turn, is converted to PCs, the Cd-induced increase inγ-ECS expression may be responsible for the observed increase in the production of NPT. Using a quantitative reverse transcription polymerase chain reaction (RT-PCR) approach, the expression of genes encoding a putative low-affinity sulfate transporter (LAST) and a putative high-affinity sulfate transporter (HAST) was determined at the transcriptional level. The RT-PCR analysis of relative transcript amounts indicates that theLASTgene inB. napusleaves showed a constitutive expression, which was hardly affected by Cd treatment. However, treatment with 30μmol/L Cd for 2 or 3 d induced a marked increase in the expression ofLASTin roots. Transcriptional expression of theHASTgene occurred in roots, but not in leaves. The expression ofHASTonly in the roots suggests that it has a specific function in sulfate uptake from soil and that the putativeLASTmay be responsible for the transport of sulfate from the roots to the shoots, as well as for the uptake of sulfate from soil. These results indicate that changes in transcriptional expression for sulfate transporters were required for the increased demand for sulfate during Cd stress.(Managing editor:Li-HuiZHAO)
- Subjects
RUTABAGA; EFFECT of cadmium on plants; CADMIUM; GLUTAMYL-tRNA synthetase; BRASSICA; BOTANICAL chemistry
- Publication
Journal of Integrative Plant Biology, 2005, Vol 47, Issue 2, p243
- ISSN
1672-9072
- Publication type
Other
- DOI
10.1111/j.1744-7909.2005.00047.x