We found a match
Your institution may have rights to this item. Sign in to continue.
- Title
Human skin cells support thymus-independent T cell development.
- Authors
Clark, Rachael A.; Yamanaka, Kei-ichi; Mei Bai; Dowgiert, Rebecca; Kupper, Thomas S.; Bai, Mei
- Abstract
Thymic tissue has previously been considered a requirement for the generation of a functional and diverse population of human T cells. We report that fibroblasts and keratinocytes from human skin arrayed on a synthetic 3-dimensional matrix support the development of functional human T cells from hematopoietic precursor cells in the absence of thymic tissue. Newly generated T cells contained T cell receptor excision circles, possessed a diverse T cell repertoire, and were functionally mature and tolerant to self MHC, indicating successful completion of positive and negative selection. Skin cell cultures expressed the AIRE, Foxn1, and Hoxa3 transcription factors and a panel of autoantigens. Skin and bone marrow biopsies can thus be used to generate de novo functional and diverse T cell populations for potential therapeutic use in immunosuppressed patients.
- Subjects
FIBROBLASTS; CONNECTIVE tissue cells; CYTOLOGICAL techniques; TRANSCRIPTION factors; IMMUNE system; CELL populations; CELL differentiation; ANTIGENS; CELL culture; CELL receptors; COMPARATIVE studies; IMMUNOLOGICAL tolerance; LIGANDS (Biochemistry); RESEARCH methodology; MEDICAL cooperation; PROTEINS; RESEARCH; RESEARCH funding; SKIN; T cells; THYMUS; HLA-B27 antigen; EVALUATION research; PHYSIOLOGY
- Publication
Journal of Clinical Investigation, 2005, Vol 115, Issue 11, p3239
- ISSN
0021-9738
- Publication type
journal article
- DOI
10.1172/JCI24731