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- Title
Attenuated liver fibrosis in the absence of B cells.
- Authors
Novobrantseva, Tatiana I.; Majeau, Gerard R.; Amatucci, Aldo; Kogan, Sophia; Brenner, Ian; Casola, Stefano; Shlomchik, Mark J.; Koteliansky, Victor; Hochman, Paula S.; Ibraghimov, Alexander
- Abstract
Analysis of mononuclear cells in the adult mouse liver revealed that B cells represent as much as half of the intrahepatic lymphocyte population. Intrahepatic B cells (IHB cells) are phenotypically similar to splenic B2 cells but express lower levels of CD23 and CD21 and higher levels of CD5. IHB cells proliferate as well as splenic B cells in response to anti-IgM and LPS stimulation in vitro. VDJ gene rearrangements in IHB cells contain insertions of N,P region nucleotides characteristic of B cells maturing in the adult bone marrow rather than in the fetal liver. To evaluate whether B cells can have an impact on liver pathology, we compared CCl4-induced fibrosis development in B cell-deficient and wild-type mice. CCl4 caused similar acute liver injury in mutant and wild-type mice. However, following 6 weeks of CCl4 treatment, histochemical analyses showed markedly reduced collagen deposition in B cell-deficient as compared with wild-type mice. By analyzing mice that have normal numbers of B cells but lack either T cells or immunoglobulin in the serum, we established that B cells have an impact on fibrosis in an antibody- and T cell-independent manner.
- Subjects
FIBROSIS; COLLAGEN diseases; LYMPHOCYTES; MONONUCLEOSIS; CELLS; NUCLEOTIDES
- Publication
Journal of Clinical Investigation, 2005, Vol 115, Issue 11, p3072
- ISSN
0021-9738
- Publication type
Article
- DOI
10.1172/JCI24798