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- Title
Production of novel angiotensin I-converting enzyme inhibitory peptides by fermentation of marine shrimp Acetes chinensis with Lactobacillus fermentum SM 605.
- Authors
Wang, Yu-Kai; He, Hai-Lun; Chen, Xiu-Lan; Sun, Cai-Yun; Zhang, Yu-Zhong; Zhou, Bai-Cheng
- Abstract
Acetes chinensis is an underutilized shrimp species thriving in Bo Hai Gulf of China. Its hydrolysate digested with protease SM98011 has been previously shown to have high angiotensin I-converting enzyme (ACE) inhibitory activity (He et al., J Pept Sci 12:726–733, ). In this article, A. chinensis were fermented by Lactobacillus fermentum SM 605 and the fermented sauce presented high ACE inhibitory activity. The minimum IC50 value (3.37 ± 0.04 mg/mL) was achieved by response surface methodology with optimized process parameters such as fermentation time of 24.19 h, incubation temperature at 38.10°C, and pH 6.12. Three ACE inhibitory peptides are purified by ultrafiltration, gel filtration, and reverse-phase high performance liquid chromatography. Identified by mass spectrometry, their amino acid sequences are Asp-Pro, Gly-Thr-Gly, and Ser-Thr, with IC50 values of 2.15 ± 0.02, 5.54 ± 0.09, and 4.03 ± 0.10 μM, respectively. Also, they are all novel ACE inhibitory peptides. Compared with protease digestion, fermentation is a simpler and cheaper method to produce ACE inhibitory peptides from shrimp A. chinensis.
- Subjects
ANGIOTENSINS; ENZYME inhibitors; PEPTIDES; FERMENTATION; ACETES; LACTOBACILLUS; ANGIOTENSIN converting enzyme; HIGH performance liquid chromatography; AMINO acid sequence; MASS spectrometry; ULTRAFILTRATION
- Publication
Applied Microbiology & Biotechnology, 2008, Vol 79, Issue 5, p785
- ISSN
0175-7598
- Publication type
Article
- DOI
10.1007/s00253-008-1489-z