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- Title
Near-Infrared Fluorescence Imaging of EGFR-Overexpressing Tumors in the Mouse Xenograft Model Using scFv-IRDye800CW and Cetuximab-IRDye800CW.
- Authors
Amini, Abolfazl; Safdari, Yaghoub; Tash Shamsabadi, Fatemeh; Azhdarinia, Ali
- Abstract
EGFR (epidermal growth factor receptor) is overexpressed in a variety of human cancers (including squamous cell carcinoma of head and neck, colon cancer, and some breast cancers) and therefore is regarded as an ideal target for cancer therapy or imaging purposes. In the current study, we produced a scFv-based near-infrared probe (called cet.Hum.scFv-IRDye-800CW) and evaluated its ability in recognizing and imaging of EGFR-overexpressing tumors in a mouse model. Like the molecular probe consisting of its parental antibody (cetuximab, an FDA-approved monoclonal antibody) and IRD800CW, cet.Hum.scFv-IRDye-800CW was able to recognize EGFR-overexpressing tumors in mice. cet.Hum.scFv-IRDye-800CW was found to be superior to the cetuximab-based probe in imaging of mouse tumors. The tumor-to-background ratio and blood clearance rate were higher when cet.Hum.scFv-IRDye-800CW was used as an imaging probe.
- Subjects
UNITED States. Food &; Drug Administration; MONOCLONAL antibodies; CETUXIMAB; EPIDERMAL growth factor receptors; LABORATORY mice; ANIMAL disease models; FLUORESCENCE
- Publication
Molecular Imaging, 2023, p1
- ISSN
1535-3508
- Publication type
Article
- DOI
10.1155/2022/9589820