We found a match
Your institution may have rights to this item. Sign in to continue.
- Title
Genome-Wide Identification, Characterization, and Expression Profiling of Eukaryotic-Specific UBP Family Genes in Brassica rapa.
- Authors
Karamat, Umer; Tabusam, Javaria; Khan, Muhammad Khalil Ullah; Awan, Muhammad Jawad Akbar; Zulfiqar, Sumer; Du, Wenchao; Farooq, Muhammad Awais
- Abstract
Ubiquitin-specific protease (UBP) family is the largest group of deubiquitinates playing a key role in eukaryotes. Presently, we identified 41 UBP genes from Brassica rapa which were classified into three groups via phylogenetic analysis. Structural and motif analysis revealed that these genes have diverse exon–intron structures and share an equal number of conserved motifs within the same group. Cis-regulatory elements were identified for salicylic acid, abscisic acid, gibberellin, methyl jasmonate, auxin, low temperature, anaerobic, defense and stress, light, and drought. Furthermore, six br-miRNAs named br-miRN159a, br-miRN159b, br-miRN159c, br-miRN159d, br-miRN324a, and br-miRN324b were found targeting BrUBP13.1, BrUBP13.2, BrUBP12.1, BrUBP12.2, BrUBP12.3, and BrUBP17. BrUBP genes were highly expressed in stem, root, silique, flower, leaf, and callus tissues; however, leaf qRT-PCR results indicated that the genes expression was high under SA and drought stresses. Our findings provide the novel foundations for future genetic and physiological studies of BrUBPs in Brassica rapa.
- Subjects
GENE families; DEUBIQUITINATING enzymes; BRASSICA; ABSCISIC acid; SALICYLIC acid; PLASMODIOPHORA brassicae; GIBBERELLINS
- Publication
Journal of Plant Growth Regulation, 2023, Vol 42, Issue 6, p3552
- ISSN
0721-7595
- Publication type
Article
- DOI
10.1007/s00344-022-10820-0