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- Title
The Importance of a Syngeneic Glioma Implantation Model: Comparison of the F98 Cell Line in Fischer and Long-Evans Rats.
- Authors
Mathieu, David; Lamarche, Jacques B.; Fortin, David
- Abstract
Objective: Certain characteristics are requited in order to improve the validity of glioma animal models. an essential one being syngeneicity. The present study was designed lo investigate the difference in inflammatory response evoked by implantation of the F98 cell line in its syngeneic host (the Fischer rat) compared to an allogeneic host (Long-Evans rat). Methods: The F98 cell line was cultured in monolayer and stereotactically implanted in the right frontal lobe of 18 Fischer rats and 18 Long-Evans rats. Animals were sacrificed when symptomatic or at 30 days from the implantation, whichever came first. H&E staining and immunocytochemistry for GFAP, CD3, and CD45 were performed on the brain specimens. The difference in the number of cells labeling for CD3 between both rat strains was used as the basis for the comparison, and was correlated with the rate of tumor take between both groups. Results: Tumor take was observed in 100% of the Fischer rats, compared to only 50% of the Long-Evans rats (P = 0.0004). Immune response was profuse in the Long-Evans rats, with a mean of 29.1 CD3-staining cells per high-power field, compared to 2.8 in the Fischer rats (P < 0.01). Conclusion: In this study, the use of an allogeneic glioma model was associated with an important inflammatory response, presumably responsible for the low tumor-take observed in this group. We believe that future in vivo studies should ideally be carried out using syngeneic implantation models. The F98/Fischer model seems to be adequate for this purpose.
- Subjects
GLIOMAS; INFLAMMATION; CELL lines; MONOMOLECULAR films; NERVOUS system tumors
- Publication
Journal of Applied Research, 2005, Vol 5, Issue 1, p17
- ISSN
1537-064X
- Publication type
Article