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- Title
IDENTIFICATION OF ASPERGILLI ISOLATED FROM DRIED WINE FRUITS FOR THE PRODUCTION OF "VIN SANTO".
- Authors
Scala, A.; Parissi, N.; Comparini, C.; Fanelli, C.; Reverberi, M.; Ricelli, A.
- Abstract
Aspergillus carbonarius, A. niger and A. tubingensis are known to produce ochratoxin A (OTA), a secondary metabolite with very dangerous effects in animals and in humans. The International Agency for Research on Cancer has classified OTA as a possible carcinogen to humans (group 2B). As a consequence, the European Commission has imposed regulatory limits for the maximum tolerable presence of this toxin in different foodstuffs. After cereals, grape products are accounted as a considerable source of human OTA intake. Based on the fungal requirements of environmental temperature and relative humidity, it is easy to suppose that wines, as the Tuscan "Vin Santo", obtained from grapes partially dried for several months to concentrate sugar content to at least 30% (w/v), are potentially at risk more than table wines. In the present work, performed in the farm "Azienda Agricola Montepaldi srl" (San Casciano Val di Pesa, Florence), we isolated from grape berries about 6x107 colony-forming units divided into six major fungal morphotypes. Among those were 24 typical colonies of Aspergillus spp. These colonies were processed in parallel using: (i) conventional culture methods that allowed their morphological description; (ii) a molecular approach based on the sequencing of the internal transcribed spacer (ITS) region. All the Aspergilli seem to belong to the species A. tubingensis. The sequencing of the ß-tubulin and calmodulin genes is in progress. We also report the amount of OTA produced in vitro by these 24 isolates.
- Subjects
ITALY; OCHRATOXINS; ASPERGILLUS toxins; ASPERGILLUS; FRUIT wines; CARCINOGENS; EUROPEAN Commission; WINES
- Publication
Journal of Plant Pathology, 2012, Vol 94, Issue 4, pS4.78
- ISSN
1125-4653
- Publication type
Article