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- Title
Remapping of the stripe rust resistance gene <italic>Yr10</italic> in common wheat.
- Authors
Yuan, Cuiling; Wu, Jingzheng; Yan, Baiqiang; Hao, Qunqun; Zhang, Chaozhong; Lyu, Bo; Ni, Fei; Caplan, Allan; Wu, Jiajie; Fu, Daolin
- Abstract
Key message: <italic>Yr10</italic> is an important gene to control wheat stripe rust, and the search for <italic>Yr10</italic> needs to be continued.Abstract: Wheat stripe rust or yellow rust is a devastating fungal disease caused by <italic>Puccinia striiformis</italic> f. sp. <italic>tritici</italic> (<italic>Pst</italic>). Host disease resistance offers a primary source for controlling wheat stripe rust. The stripe rust resistance gene <italic>Yr10</italic> confers the race-specific resistance to most tested <italic>Pst</italic> races in China including CYR29. Early studies proposed that <italic>Yr10</italic> was a nucleotide-binding site, leucine-rich repeat gene archived as GenBank accession AF149112 (hereafter designated the <italic>Yr10</italic> candidate gene or <italic>Yr10</italic><italic>CG</italic>). In this study, we revealed that 15 Chinese wheat cultivars positive for <italic>Yr10</italic><italic>CG</italic> are susceptible to CYR29. We then expressed the <italic>Yr10</italic><italic>CG</italic> cDNA in the common wheat ‘Bobwhite’. The <italic>Yr10</italic><italic>CG</italic>-cDNA positive transgenic plants were also susceptible to CYR29. Thus, it is highly unlikely that <italic>Yr10</italic><italic>CG</italic> corresponds to the <italic>Yr10</italic> resistance gene. Using the <italic>Yr10</italic> donor ‘Moro’ and the <italic>Pst</italic>-susceptible wheat ‘Huixianhong’, we generated two F3 populations that displayed a single Mendelian segregation on the <italic>Yr10</italic> gene, and used them to remap the <italic>Yr10</italic> gene. Six markers were placed in the <italic>Yr10</italic> region, with the <italic>Yr10</italic><italic>CG</italic> gene now mapping about 1.2-cM proximal to the <italic>Yr10</italic> locus and the <italic>Xsdauw79</italic> marker is completely linked to the <italic>Yr10</italic> locus. Apparently, the <italic>Yr10</italic> gene has not yet been identified. Fine mapping and positional cloning of <italic>Yr10</italic> is important for gene pyramiding for stripe rust resistance in wheat.
- Subjects
WHEAT genetics; VEGETATION mapping; STRIPE rust; NUCLEOTIDE sequence; ANTISENSE DNA
- Publication
Theoretical & Applied Genetics, 2018, Vol 131, Issue 6, p1253
- ISSN
0040-5752
- Publication type
Article
- DOI
10.1007/s00122-018-3075-9