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- Title
Fine mapping of Co- x, an anthracnose resistance gene to a highly virulent strain of Colletotrichum lindemuthianum in common bean.
- Authors
Richard, Manon; Pflieger, Stéphanie; Sévignac, Mireille; Thareau, Vincent; Blanchet, Sophie; Li, Yupeng; Jackson, Scott; Geffroy, Valérie
- Abstract
Key message: The Co - x anthracnose R gene of common bean was fine-mapped into a 58 kb region at one end of chromosome 1, where no canonical NB-LRR-encoding genes are present in G19833 genome sequence. Abstract: Anthracnose, caused by the phytopathogenic fungus Colletotrichum lindemuthianum, is one of the most damaging diseases of common bean, Phaseolus vulgaris. Various resistance (R) genes, named Co-, conferring race-specific resistance to different strains of C. lindemuthianum have been identified. The Andean cultivar JaloEEP558 was reported to carry Co- x on chromosome 1, conferring resistance to the highly virulent strain 100. To fine map Co- x, 181 recombinant inbred lines derived from the cross between JaloEEP558 and BAT93 were genotyped with polymerase chain reaction (PCR)-based markers developed using the genome sequence of the Andean genotype G19833. Analysis of RILs carrying key recombination events positioned Co- x at one end of chromosome 1 to a 58 kb region of the G19833 genome sequence. Annotation of this target region revealed eight genes: three phosphoinositide-specific phospholipases C (PI-PLC), one zinc finger protein and four kinases, suggesting that Co- x is not a classical nucleotide-binding leucine-rich encoding gene. In addition, we identified and characterized the seven members of common bean PI-PLC gene family distributed into two clusters located at the ends of chromosomes 1 and 8. Co- x is not a member of Co- 1 allelic series since these two genes are separated by at least 190 kb. Comparative analysis between soybean and common bean revealed that the Co- x syntenic region, located at one end of Glycine max chromosome 18, carries Rhg1, a major QTL contributing to soybean cyst nematode resistance. The PCR-based markers generated in this study should be useful in marker-assisted selection for pyramiding Co- x with other R genes.
- Subjects
PLANT gene mapping; ANTHRACNOSE; COLLETOTRICHUM lindemuthianum; COMMON bean; GENETIC code; PHYTOPATHOGENIC fungi
- Publication
Theoretical & Applied Genetics, 2014, Vol 127, Issue 7, p1653
- ISSN
0040-5752
- Publication type
Article
- DOI
10.1007/s00122-014-2328-5