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- Title
花生脂肪酸延长酶基因AhFAE1及其启动子的克隆与功能分析.
- Authors
石磊; 黄冰艳; 齐飞艳; 苗利娟; 刘华; 张忠信; 高伟; 董文召; 汤丰收; 张新友
- Abstract
In order to explore the reasons for low erucic acid content in peanut seeds, FAE1 cDNA and its promoter sequence were isolated and their functionality were analyzed. Results showed that a full-length cDNA sequence and its promoter were isolated. Sequence analysis showed that cDNA fragment length was 2202 bp containing 5'-untranslated region of 441 bp, and 3'-untranslated region of 201 bp, encoding a protein of 517 amino acids with a predicted molecular mass of 58.17 Da and theoretical pI of 9.15. Phylogenetic tree analysis revealed that AhFAE1 shared closer genetic relationship with homologues from Jatropha curcas (ALB76796.1), Corchorus capsularis (OMO87584.1) and Arabidopsis (AtKCS4). Subcellular localization assays showed that AhFAE1 protein was located in Endoplasmic Reticulum. qRT-PCR revealed that AhFAE1 transcripts were constitutively expressed in all examined tissues and displayed a bell-shaped pattern throughout seed development with expression peaking at 60 DAF. To study the function of this promoter, we constructed a binary plant vector pBI-PAhFAE1, which was introduced into Arabidopsis by Agrobacterium-mediated transformation. Histochemical staining analysis indicated that no GUS activity was detected in any tissue. AhFAE1 was potentially involved in very long chain fatty acid synthesization. Low transcriptional activity of AhFAE1 promoter could have caused the low content of erucic acid in peanut.
- Subjects
MOLECULAR cloning; MOLECULAR weights; JATROPHA; SEED development; ENDOPLASMIC reticulum; ANTISENSE DNA; SEQUENCE analysis
- Publication
Chinese Journal of Oil Crop Sciences, 2019, Vol 41, Issue 2, p176
- ISSN
1007-9084
- Publication type
Article
- DOI
10.7505/j.issn.1007-9084.2019.02.003